Efficacy of tetrasodium-EDTA and silver alone and in a complex with metal ions against mono species biofilms.

Abstract: Biofilms can form on abiotic and biotic surfaces, such as medical devices and wounds causing infection and delayed wound healing. Biofilms often show increased tolerance to antimicrobials and antibiotics in comparison to their planktonic counterparts and are therefore difficult to treat. Hypothesis and aims: The aim of this study was to evaluate antibiofilm activity of several Tetrasodium-EDTA (TEDTA) complexes against mono and mixed species biofilms. Methodology: The minimum biofilm eradication concentration (MBEC) was determined following an adapted version of ASTM E2799 17 against mono and mixed species biofilms of Acinetobacter baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 29213 and Staphylococcus epidermidis ATCC 35984. The biofilm MIC and MBEC were determined visually as the lowest concentration of complex to inhibit biofilm growth/dissemination and that to eradicate the biofilm, respectively. Results: Following MBEC testing of TEDTA alone and in complexes with various metal ions, increased antibiofilm potency was found with the complexes. The complexes demonstrated good antibiofilm activity against mono and mixed species biofilms. Conclusion: The results demonstrate the potential for the TEDTA complexes to be used for treatment of biofilms, such as those that form in chronic wounds.

Publication Details: