Abstract: Biofilms are formed when bacterial cells adhere to a surface and each other and secrete extracellular polymeric substances (EPS), encasing themselves in a matrix. There is increasing evidence showing the presence of biofilms on surfaces, such as wounds, can lead to infection, inflammation and delayed wound healing. Hypothesis and aims: The aim of this study was to evaluate a Tetrasodium-EDTA complex-polymer against a Pseudomonas aeruginosa biofilm formed on various platforms using the drip flow bioreactor model. Methodology: Two models were developed using different surfaces and immature (24 H) and mature (48 H) biofilms of Pseudomonas aeruginosa. The first model was set up in accordance with ASTM E2647 13. This involved growing a biofilm for 48 H on a glass coupon. The second model was set up using adsorbent pads and membrane filters. Tetrasodium-EDTA complex-polymers were applied for 24 H before neutralising and either scraping or sonicating the samples. Biofilm density was determined by performing serial dilutions and plating onto agar. Results: Tetrasodium-EDTA complex-polymers demonstrated antibiofilm activity against an immature and mature biofilm of P. aeruginosa in the drip flow bioreactor model, showing a significant log reduction in biofilm cell density. Conclusion: Tetrasodium-EDTA complex-polymers demonstrated efficacy against P. aeruginosa biofilms and should be investigated further for treatment of acute and chronic wounds.
Publication Details:
Author(s): Salisbury, A-M; Mullin, M.; Foulkes, L.; Chen, R.; Percival, S.L.
Year: Eurobiofilms 2019. Glasgow, UK, 03-06, September, 2019.
Edition:
ISBN:
