Abstract: Aim: Biofilms targeted silver-cerium EDTA complexes (Ag-Ce MMC) were formulated and their antibacterial/antibiofilm efficacies were evaluated on different in vitro models. Method: A variety of in vitro microbial and biofilm models were utilized to evaluate the ability of Ag-Ce MMC in different platforms (liquid/gel) to sequester microbes, prevent dissemination, and manage bioburden. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Ag-Ce MMC and it’s the mixtures with surfactant Poloxamer P188 and P407 were determined using a broth microdilution method against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. CDC bioreactor, drip-flow model, contact lens model and artificial wound slough biofilm (AWS-biofilm) models were used to evaluate antibacterial killing efficacy towards established P. aeruginosa and Staphylococcus aureus biofilms. Results / Discussion: Following MIC and MBC testing of Ag-Ce MMC and the components alone, increased antimicrobial potency was found with all complexes tested. Checkerboard assays showed synergistic interactions between Ag-Ce MMC with poloxamer P188 and P407 against P. aeruginosa, S. aureus, and C. albicans. CDC, drip-flow model, contact lens model and AWS-biofilm model demonstrated strong antibiofilm activity of Ag-Ce MMC against P. aeruginosa and S. aureus. The Ag-Ce MMC loaded gels (Carbopol and poloxamer) demonstrated superiority in both CDC and AWS biofilm models, as reduced P. aeruginosa growth to the limit of detection (completely killed). Conclusion: The data generated within the in vitro biofilm models demonstrate that the Ag-Ce MMC complexes are the promising reagents for effective biofilm management and control in wound care.
Publication Details:
Author(s): Chen R, Swann MJ, Saint Bezard J, Griffiths F, Catton R, Watson F, Percival SL.
Year: EWMA 2025, Barcelona, Spain, 26-28 March, 2025.
Edition:
ISBN:
