Abstract: Aim: Wound irrigation solutions are commonly used to prevent the colonization of implants and reduce biofilm formation on the surface. The aim of this study was to evaluate a novel antimicrobial wound irrigation solutions using multifunctional metal complexes (MMC) comprising of silver, cerium, EDTA and/or DTPA against biofilm formation on titanium surfaces. Method: Biofilm prevention capabilities of Ag-Ce MMC solutions were assessed using a 12 well assay, Ti coupons were placed into 4mL of the MMC solution, then inoculated with 1mL of Staphylococcus aureus and incubated for 24 hours at 37°C, shaking at 125rpm. After 24 hours, coupons were transferred into Dey-Engley neutraliser and sonicated on full power for 30 minutes, before being serially diluted and plated out onto TSA. A 24 hour S. aureus biofilm was established on titanium coupons using a standard drip flow model, before removal of planktonic cells and exposure to the treatment solution. After being exposed for 24 hours the coupons were enumerated to confirm total viability counts and subject to microscopy using fluorescent staining. Results / Discussion: Ag-Ce MMC demonstrated biofilm prevention capabilities, when challenged with a 24 hour contact time, showing a >4 log reduction when compared to the untreated control (NaCl 0.9%). Ag-Ce MMC also demonstrated potent antibiofilm activity against S. aureus in the drip flow biofilm model. Conclusion: The results outlined in this study suggest that Ag-Ce MMC could be used in the clinic to prevent biofilm formation or eradicate pre-formed biofilms, such as those in the wound or on titanium implants.
Publication Details:
Author(s): Catton R, Watson F, Griffiths F, Saint Bezard J, Chen R, Swann MJ, Percival SL.
Year: EWMA 2025, Barcelona, Spain, 26-28 March,2025.
Edition:
ISBN:
