T-edta complexes synthesis and in vitro cytotoxicity test.

Abstract: In this study, we synthesized ten t-EDTA complexes with different concentration of metals (copper, zinc and cerium) and we also examined the cytotoxicity of t-EDTA complexes compared to some commercial antimicrobial wound dressing in an in vitro direct contact cytotoxic assay model. The t-EDTA complexes (C1 to C10) were synthesized by the reactions of t-EDTA with one or two selected metal salts at pre-defined concentrations. The in vitro cytotoxicity of the complexes was measured by direct contact assay on fibroblasts (L929) and human dermal fibroblasts (HDFa). Briefly, L929 and HDFa were seeded into 24 well plate at concentration of 5×104/ml/well. After 24 h culture, t-EDTA complexes were added into pre-determined wells at a concentration equivalent to the MIC against P. aeruginosa ATCC 15442 (the results not shown here). After 24 h, cell images were taken, and the cells were washed with PBS and stored in -80C. The cell viability of L929 and HDFa was measured using commercially available CyQUANT cell proliferation assay. The cell viability of HDFa was higher than 80% after t-EDTA/silver/zinc treatments at MIC concentrations, which means t-EDTA/silver/zinc complexes are non-cytotoxic. T-EDTA/silver/copper and t-EDTA/silver/cerium complexes were cytotoxic (cell viability < 70%). However, cell viability after treatment with all the complexes was higher than cell viability following treatment with other commercial antimicrobial wound dressings. The results demonstrated that compared to some commercial antibacterial wound dressings, t-EDTA complexes are less cytotoxic. The results indicated that t-EDTA complexes are novel promising chemicals for manufacture next generation wound healing dressings, with anti-biofilm, anti-microbial and anti-inflammatory activities.

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