Abstract: There is increasing evidence showing the presence of biofilms on surfaces, such as wounds, leading to infection, inflammation and delayed wound healing. Biofilms often show increased tolerance to antimicrobials and antibiotics in comparison to their planktonic counterparts and are therefore difficult to treat. The aim of this study was to evaluate antimicrobial and antibiofilm activity of several Tetrasodium-EDTA (TEDTA) complexes against common wound pathogens. The minimum inhibitory concentration (MIC) of TEDTA alone and in several different complexes with silver and various metal ions was determined using a broth microdilution method. The minimum biofilm eradication concentration (MBEC) was determined following ASTM E2799 17. Synergistic interactions between components of the complexes was evaluated using a 2D checkerboard MIC method and determining the fractional inhibitory concentration index (FICI). Following MIC and MBEC testing of TEDTA alone and in complexes with silver and various metal ions, increased antimicrobial and antibiofilm potency was found with all complexes tested. Checkerboard assays showed synergistic interactions between several silver compounds and TEDTA, with an FICI of 0.2 – 0.5 against Pseudomonas aeruginosa and Staphylococcus aureus. The results show the potential for TEDTA complexes to be used for difficult to treat wound infections.
Publication Details:
Author(s): Salisbury, A-M; Mullin, M.; Tyldesley, H.; Chen, R.; Percival, S.L.
Year: SAWC 2019 spring. San Antonio, Texas, from May 7-11, 2019.
Edition:
ISBN:
