The in vitro cytotoxicity study on novel t-EDTA complexes.

Abstract: Tetrasodium EDTA (t-EDTA) has been shown to be an essential tool in management of biofilm-related infections and should be considered as an anti-biofilm agent alone or in combination with other antimicrobials or technologies for increased antimicrobial performance in recalcitrant wounds. Hypothesis and aims: T-EDTA can form different complexes with more than one metal ion by either covalent bond, chelation or ionic bond. In this study, we examined the cytotoxicity of a novel series of t-EDTA complexes in an in vitro model. Methodology: Ten t-EDTA complexes (C1 to C10) were synthesized by the reactions of t-EDTA with one or two selected metal salts at pre-defined concentrations. The synthesized complexes were characterized by FTIR, MS and PXRD. The in vitro cytotoxicity of the complexes was measured by direct contact assay on fibroblasts (L929) and human dermal fibroblasts (HDFa). Briefly, L929 and HDFa were seeded into 24 well plates at a concentration of 5×104/ml/well. After 24 h culture, t-EDTA complexes were added into pre-determined wells at a concentration equivalent to the MIC against P. aeruginosa ATCC 15442. After 24 h, cell images were taken, and the cells were washed with PBS and stored at -80°C. The cell viability of L929 and HDFa was measured using commercially available CyQUANT cell proliferation assay. Results: The cell viability of HDFa was higher than 80% after C2, C3 and C4 treatment at MIC concentrations, which means these complexes are non-cytotoxic at an efficacious concentration. C5, C6, C7, C8, C9 and C10 complexes were cytotoxic (cell viability < 70%), however; cell viability after treatment with all the complexes was higher than cell viability following treatment with other commercial antimicrobial wound dressings. Conclusion: Compared to some commercial wound dressings, t-EDTA complexes are less cytotoxic. The results indicate that t-EDTA complexes are novel, promising chemicals for next generation wound healing dressings, with anti-biofilm, anti-microbial and anti-inflammatory activities.

Publication Details: